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mouse anti cd104  (R&D Systems)


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    Structured Review

    R&D Systems mouse anti cd104
    Mouse Anti Cd104, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd104/Human+Integrin+beta+4%2FCD104+Antibody/pm41735293-593-25-27
    Average 93 stars, based on 10 article reviews
    mouse anti cd104 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Extracellular Vesicle Antibody Microarray for Multiplexed Inner and Outer Protein Analysis
    Article Snippet: The following trios of barcoded antibodies were used for triplexed detection: CD63-BC38, ITG α2 (MAB1233, R&D Systems)-BC64 and HSP70-BC23; CD9-BC51, ITG α6 (MAB1350, R&D Systems)-BC09 and Alix (MA1-83977, Invitrogen)-BC60; CD81-BC50, ITG β1 (MAB17781, R&D Systems)-BC27, HSP90-BC60; CD82 (342102, Biolegend)-BC43, ITG β4 (MAB4060, R&D Systems)-BC79, claudin-2 (32-5600, ThermoFisher)-BC61.

    Article Title: Spatiotemporal dynamics exhibited by horizontal basal cells reveal a pro-neurogenic pathway during injury-induced olfactory epithelium regeneration
    Article Snippet: Antibody Source Identifier Dilution Antigen Retrieval Required Signal Detection Mouse αp63 ATCC Clone 4A4 1:100 Yes Secondary fluorophore Mouse αEzrin Developmental Studies Hybridoma Bank CPTCEzrin-1 1:25 No SAfluorophore Rabbit αLaminin Novus Biologicals NB300144 1:100 No Secondary fluorophore Goat αItgb1 R&D Systems AF2405 1:13 No Secondary fluorophore Rat α-Itgb4 R&D Systems MAB4054 1:20 No Secondary fluorophore Chicken αCK5 BioLegend Clone Poly9059 1:200 No Secondary fluorophore Rabbit αRac1 Proteintech 24072-1- AP 1:200 Yes TSA Rabbit αpFAKY925 Cell Signaling Technology 3284 1:100 Yes TSA Rabbit αpcJun Cell Signaling Technology 3270 1:40 Yes TSA Rabbit αPGP9.5 Proteintech 14730-1- AP 1:200 Yes Secondary fluorophore

    Article Title: Extracellular Vesicle Antibody Microarray for Multiplexed Inner and Outer Protein Analysis.
    Article Snippet: The following trios of barcoded antibodies were used for triplexed detection: CD63-BC38, ITG α2 (MAB1233, R&D Systems)-BC64, and HSP70-BC23; CD9-BC51, ITG α6 (MAB1350, R&D Systems)-BC09, and Alix (MA1-83977, Invitrogen)-BC60; CD81-BC50, ITG β1 (MAB17781, R&D Systems)-BC27, and HSP90-BC60; CD82 (342102, Biolegend)-BC43, ITG β4 (MAB4060, R&D Systems)-BC79, and claudin-2 (32−5600, Thermo Fisher)-BC61.

    Blocking Assay:

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slides were blocked with blocking solution for 30 minutes at RT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) and mouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500 μg ml -1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and anti-mouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis.
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slideswereblockedwith blocking solution for 30minutes atRT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) andmouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500μgml-1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and antimouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Incubation:

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slides were blocked with blocking solution for 30 minutes at RT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) and mouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500 μg ml -1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and anti-mouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis.
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slideswereblockedwith blocking solution for 30minutes atRT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) andmouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500μgml-1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and antimouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Bioprocessing:

    Article Title: Integrins are enriched on aberrantly fucosylated tumour‐derived urinary extracellular vesicles
    Article Snippet: RPMI-1640 were purchased from Lonza (Belgium) and 10% fetal bovine serum (FBS) was acquired from GIBCO (USA). .. Monoclonal antibodies anti-ITGA1 (clone 639508) (catalog # MAB5676), -ITGA2 (clone HAS3) (catalog # MAB1233), - ITGA3 (clone IA3) (catalog # MAB1345), -ITGA5 (clone 612557) (catalog # MAB18642), -ITGA6 (clone MP4F10) (catalog # MAB1350), -ITGAV (clone 273210) (catalog #MAB1219), -ITGAVB3 (clone 23C6) (catalog #MAB3050), -ITGB1 (4B7R) (catalog #MAB1778), -ITGB4 (clone 422325) (catalog #MAB4060) and -CD9 (clone 209306) (catalog #MAB1880) were purchased from R&D systems (Abingdon, UK). .. Other monoclonal antibodies anti-ITGAM (clone ICRF44) (catalog # 555386), -ITGAX (clone 3.9) (catalog # 565805), -ITGB2 (clone 6.7) (catalog # 555922), -ITGB3 (clone VI-PL2) (catalog # 555752), and -CD63 (clone 556019) (catalog # 556019) were purchased from BD Bioscience (Vantaa, Finland).

    Article Title: Integrins are enriched on aberrantly fucosylated tumour‐derived urinary extracellular vesicles
    Article Snippet: RPMI‐1640 were purchased from Lonza (Belgium) and 10% fetal bovine serum (FBS) was acquired from GIBCO (USA). .. Monoclonal antibodies anti‐ITGA1 (clone 639508) (catalog # MAB5676), ‐ITGA2 (clone HAS3) (catalog # MAB1233), ‐ITGA3 (clone IA3) (catalog # MAB1345), ‐ITGA5 (clone 612557) (catalog # MAB18642), ‐ITGA6 (clone MP4F10) (catalog # MAB1350), ‐ITGAV (clone 273210) (catalog # MAB1219), ‐ITGAVB3 (clone 23C6) (catalog # MAB3050), ‐ITGB1 (4B7R) (catalog # MAB1778), ‐ITGB4 (clone 422325) (catalog # MAB4060) and ‐CD9 (clone 209306) (catalog # MAB1880) were purchased from R&D systems (Abingdon, UK). .. Other monoclonal antibodies anti‐ITGAM (clone ICRF44) (catalog # 555386), ‐ITGAX (clone 3.9) (catalog # 565805), ‐ITGB2 (clone 6.7) (catalog # 555922), ‐ITGB3 (clone VI‐PL2) (catalog # 555752), and ‐CD63 (clone 556019) (catalog # 556019) were purchased from BD Bioscience (Vantaa, Finland).

    Cell Culture:

    Article Title: 3D bioprinting of an implantable xeno‐free vascularized human skin graft
    Article Snippet: .. ECs, FBs, PCs, and KCs cultured under xeno‐free conditions were analyzed for surface and intracellular markers expression by flow cytometry and immunofluorescence microscopy using antibodies against: CD31 (WM59; Biolegend), CD45 (2D1; Biolegend), ZO‐1 (sc‐33725; Santa Cruz), VE‐cadherin (sc‐6458; Santa Cruz), vWF (ab201336; abcam) claudin‐5 (34‐1600; Invitrogen), PDGFR‐α (16A1; Biolegend), PDGFR‐β (18A2; Biolegend), CD90 (5E10; Biolegend), NG2 (9.2.27; Invitrogen), a‐SMA (1A4; Invitrogen), FAP (AF3715; Novus Biologics), integrins α2β1 (ab24697; abcam), α5β1 (NBP2‐52680; Novus Biologics), αvβ3 (sc‐7312; Santa Cruz), α6 (MAB13501; R&D systems), α3 (MAB1345; R&D systems), β4 (NBP1‐43369), CK14 (LL002; Novus Biologics), CK10 (EP1607IHCY, abcam), and occludin. ..

    Expressing:

    Article Title: 3D bioprinting of an implantable xeno‐free vascularized human skin graft
    Article Snippet: .. ECs, FBs, PCs, and KCs cultured under xeno‐free conditions were analyzed for surface and intracellular markers expression by flow cytometry and immunofluorescence microscopy using antibodies against: CD31 (WM59; Biolegend), CD45 (2D1; Biolegend), ZO‐1 (sc‐33725; Santa Cruz), VE‐cadherin (sc‐6458; Santa Cruz), vWF (ab201336; abcam) claudin‐5 (34‐1600; Invitrogen), PDGFR‐α (16A1; Biolegend), PDGFR‐β (18A2; Biolegend), CD90 (5E10; Biolegend), NG2 (9.2.27; Invitrogen), a‐SMA (1A4; Invitrogen), FAP (AF3715; Novus Biologics), integrins α2β1 (ab24697; abcam), α5β1 (NBP2‐52680; Novus Biologics), αvβ3 (sc‐7312; Santa Cruz), α6 (MAB13501; R&D systems), α3 (MAB1345; R&D systems), β4 (NBP1‐43369), CK14 (LL002; Novus Biologics), CK10 (EP1607IHCY, abcam), and occludin. ..

    Flow Cytometry:

    Article Title: 3D bioprinting of an implantable xeno‐free vascularized human skin graft
    Article Snippet: .. ECs, FBs, PCs, and KCs cultured under xeno‐free conditions were analyzed for surface and intracellular markers expression by flow cytometry and immunofluorescence microscopy using antibodies against: CD31 (WM59; Biolegend), CD45 (2D1; Biolegend), ZO‐1 (sc‐33725; Santa Cruz), VE‐cadherin (sc‐6458; Santa Cruz), vWF (ab201336; abcam) claudin‐5 (34‐1600; Invitrogen), PDGFR‐α (16A1; Biolegend), PDGFR‐β (18A2; Biolegend), CD90 (5E10; Biolegend), NG2 (9.2.27; Invitrogen), a‐SMA (1A4; Invitrogen), FAP (AF3715; Novus Biologics), integrins α2β1 (ab24697; abcam), α5β1 (NBP2‐52680; Novus Biologics), αvβ3 (sc‐7312; Santa Cruz), α6 (MAB13501; R&D systems), α3 (MAB1345; R&D systems), β4 (NBP1‐43369), CK14 (LL002; Novus Biologics), CK10 (EP1607IHCY, abcam), and occludin. ..

    Immunofluorescence:

    Article Title: 3D bioprinting of an implantable xeno‐free vascularized human skin graft
    Article Snippet: .. ECs, FBs, PCs, and KCs cultured under xeno‐free conditions were analyzed for surface and intracellular markers expression by flow cytometry and immunofluorescence microscopy using antibodies against: CD31 (WM59; Biolegend), CD45 (2D1; Biolegend), ZO‐1 (sc‐33725; Santa Cruz), VE‐cadherin (sc‐6458; Santa Cruz), vWF (ab201336; abcam) claudin‐5 (34‐1600; Invitrogen), PDGFR‐α (16A1; Biolegend), PDGFR‐β (18A2; Biolegend), CD90 (5E10; Biolegend), NG2 (9.2.27; Invitrogen), a‐SMA (1A4; Invitrogen), FAP (AF3715; Novus Biologics), integrins α2β1 (ab24697; abcam), α5β1 (NBP2‐52680; Novus Biologics), αvβ3 (sc‐7312; Santa Cruz), α6 (MAB13501; R&D systems), α3 (MAB1345; R&D systems), β4 (NBP1‐43369), CK14 (LL002; Novus Biologics), CK10 (EP1607IHCY, abcam), and occludin. ..

    Microscopy:

    Article Title: 3D bioprinting of an implantable xeno‐free vascularized human skin graft
    Article Snippet: .. ECs, FBs, PCs, and KCs cultured under xeno‐free conditions were analyzed for surface and intracellular markers expression by flow cytometry and immunofluorescence microscopy using antibodies against: CD31 (WM59; Biolegend), CD45 (2D1; Biolegend), ZO‐1 (sc‐33725; Santa Cruz), VE‐cadherin (sc‐6458; Santa Cruz), vWF (ab201336; abcam) claudin‐5 (34‐1600; Invitrogen), PDGFR‐α (16A1; Biolegend), PDGFR‐β (18A2; Biolegend), CD90 (5E10; Biolegend), NG2 (9.2.27; Invitrogen), a‐SMA (1A4; Invitrogen), FAP (AF3715; Novus Biologics), integrins α2β1 (ab24697; abcam), α5β1 (NBP2‐52680; Novus Biologics), αvβ3 (sc‐7312; Santa Cruz), α6 (MAB13501; R&D systems), α3 (MAB1345; R&D systems), β4 (NBP1‐43369), CK14 (LL002; Novus Biologics), CK10 (EP1607IHCY, abcam), and occludin. ..



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    Image Search Results


    Journal: eLife

    Article Title: The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis

    doi: 10.7554/eLife.86931

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-integrin β4/CD104 (rat monoclonal 439-9B) , eBioscience , 14-1049-82 RRID: AB_1210460 , FACS (1:100).

    Techniques: Plasmid Preparation, Control, Sequencing, Negative Control, Recombinant, Software, Transfection, Histopathology, Western Blot, Staining, Permeability

    Journal: eLife

    Article Title: The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis

    doi: 10.7554/eLife.86931

    Figure Lengend Snippet:

    Article Snippet: Antibodies were Δ-HS (F69-3G10, AMS Biotechnology), CS (CS56, Merck), HSPG2/perlecan (7B5, ThermoFisher Scientific), glypican-1 (AF4519), integrin β4/CD104 (clone 439-9B, eBioscience), integrin β1/CD29 (P4C10, NBP2-36561), syndecan-2 (MAB2965), biglycan (AF2667), laminin α5 (NBP2-42391) from Biotechne.

    Techniques: Plasmid Preparation, Control, Sequencing, Negative Control, Recombinant, Software, Transfection, Histopathology, Western Blot, Staining, Permeability